363 Age-dependent changes in lipidome of autophagy-deficient mice

Previous studies with mice lacking epidermal macroautophagy suggest that under pro-aging stress there is a profound change in lipid metabolism and an increased susceptibility to DNA damage cellular senescence. Thus we followed up these now cohorts of young (< 8 mo) geriatric (>16 which did or not lack Atg7 (Atg7 f/f: K14 cre+), studied the consequences chronological aging on level lipidome. Additionally, analyzed transcriptome corresponding for genes function signaling differentially expressed age and/or autophagy deficiency. While lipidomic analysis showed significantly triacylglycerols (TAG) affecting all identified TAG species, this increase was lower old cre+ compared WT, suggesting deficiency might age-related storage lipids. Among other investigated classes, sphingomyelins (SM) cholesteryl esters (CE) were decreased both deficient mice. Decreased respective control groups PUFA species eicosapentaenoate, -arachidonate –docosahexaenoate. Transcriptomic revealed significant upregulation fatty-acid binding protein 5 (FABP5) aged Atg7-defificient mice, could influence composition of- through The observed FABP5 would be compatible lipolysis thus reduced accumulation Atg7-deficient nuclear translocation epidermis proinflammatory gene expression, e.g. CXCL3, PTGES epidermis. Our results autophagy-deficiency affects lipids but expression specific genes.